Workflow tutorials
ΒΆ
Variant sorting screen tutorial
Example workflow
1. Count gRNA & reporter (
count_samples
)
(Optional) Profile editing pattern (
profile
)
2. QC samples & guides (
qc
)
3. Quantify variant effect (
run
)
Tiling sorting screen tutorial
Example workflow
1. Count gRNA & reporter (
count_samples
)
(Optional) Profile editing pattern (
profile
)
2. QC (
qc
)
3. Filter alleles (
filter
)
4. Quantify variant effect (
run
)
Variant survival screen tutorial
Example workflow
1. Count gRNA & reporter (
count_samples
)
(Optional) Profile editing pattern (
profile
)
2. QC samples & guides (
qc
)
3. Quantify variant effect (
run
)
Tiling survival screen tutorial
Example workflow
1. Count gRNA & reporter (
count_samples
)
(Optional) Profile editing pattern (
profile
)
2. QC (
qc
)
3. Filter alleles (
filter
)
4. Quantify variant effect (
run
)
Sorting screen tutorial without reporter edits
Example workflow
Input file spec
Visualize result (variant screens)
Feeding custom prior into
bean
run
Example workflow
Manually specifying prior
bean
Activity-normalized variant effect size estimation from pooled CRISPR screens
Table of Contents
Workflow tutorials
Variant sorting screen tutorial
Tiling sorting screen tutorial
Variant survival screen tutorial
Tiling survival screen tutorial
Sorting screen tutorial without reporter edits
Visualize result (variant screens)
Feeding custom prior into
bean
run
BEAN model description
Input file format
Subcommands
CRISPR Reporter Screen analysis with BEAN
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