CRISPRlungo

Inputs

Treated FASTQ

.fastq / .fq / .gz

(.fastq /.fq <500Mb, .gz <200Mb)

Control FASTQ

Optional for backgorund error filtering

(.fastq /.fq <500Mb, .gz <200Mb)

Protospacer (w/o PAM)

e.g. GGCGCCCTGGCCAGTCGTCT

Additional Protospacer (w/o PAM, Optional)

Optional for second gRNA.

Reference

Note: For optimal performance, a reference sequence shorter than 25 kbp is recommended on web tool.

Intended edit (Optional)

Analysis window (nt)

Whole window between targets

True False

Cleavage site in target (nt)

Large insertion length cutoff (nt)

Large deletion length cutoff (nt)

Examples

0%

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FASTQ file alignment

Allele assignments